OBJECTIVE: The aim of present study is to examine the effect of subchronic dichlorvos (DDVP) administration on fallopian tube damage and assess the preventive effects of combination of vitamins E and C against DDVP toxicity. MATERIAL-METHOD: The experimental groups were rats treated with corn oil (control group), rats treated with a dose of 8 mg/kg DDVP, and rats treated with a dose of 8 mg/kg body weight DDVP plus vitamins E and C (DDVP +Vit). The groups were given DDVP by oral gavage on 5 days a week for 4 weeks at a daily dose of 8 mg/kg (DDVP and DDVP +Vit) using corn oil as the vehicle. Vitamins E and C were injected at doses of 50 mg/kg intramuscularly and 20 mg/kg intraperitoneally, respectively, after the treatment with DDVP in the DDVP+Vit group. The levels of malondialdehyde (MDA) were determined in the rat plasma. RESULTS: MDA levels were higher in the DDVP group than in the control group, and lower in the DDVP +Vit group than in the DDVP group Histopathological examination of fallopian tube was carried out by transmission electron microscopy. In our electron microscopic investigations DDVP administration caused the apical surface differentiations of both microvillus and kinocilliated cells to fully disappear. Moreover, specific vacuoles, mitochondrial crystallosis and swelling, and chromatin loss occured in all of the cells. Administration of Vitamin E and C along with DDVP significantly reduced the histopathological changes. CONCLUSION: From these results, it can be concluded that subchronic DDVP causes fallopian tube damage, and lipid peroxidation may be a molecular mechanism involved in DDVP-induced toxicity. Furthermore, the combination of vitamins E and C can reduce the toxic effects of DDVP on fallopian tube tissue of rats.
Keywords: dichlorvos, fallopian tube, lipid peroxidation, vitamin C, vitamin E